Journal: Biophysical Journal

Article Title: Simultaneous Release of Multiple Vesicles from Rods Involves Synaptic Ribbons and Syntaxin 3B

doi: 10.1016/j.bpj.2019.10.006

Figure Lengend Snippet: Amplitude characteristics of spontaneous IA(glu) events are consistent with multiquantal release. (A) Spontaneous multiquantal events were observed among many uniquantal events in rods voltage clamped at −70 mV. (B) Shown is a representative amplitude histogram of spontaneous rod IA(glu) events (n = 325) fit with a multiple Gaussian function. The inset shows a representative segment of the recording from which the histogram was derived. By assuming that the mean ± SD of the initial peak represents a single quantum, quantal content for this cell was calculated as a weighted average of the areas under the curve and found to be 1.69. (C) Amplitude of IA(glu) events (n = 339) was strongly correlated with event charge transfer (R = 0.99) with nonzero slope (F-test, p < 0.0001).

Article Snippet: Events within one SD of the mean of the first peak were considered “uniquantal,” and remaining larger events were considered “multiquantal.” Quantal content was calculated from weighted averages of the area under the curves from a multiple Gaussian fit (GraphPad Prism 4).

Techniques: Derivative Assay